Bioimpedance Spectra in Final RT-PCR Products: A Sensitivity Threshold Analysis

Bioimpedance Spectra in Final RT-PCR Products: A Sensitivity Threshold Analysis

Several studies have showed that Deoxyribonucleic Acids (DNA) molecule has a natural electronegative condition and its electrical properties, such as electrical bioimpedance (EBI’s), are strongly associated to DNA concentration. In this work, we propose a simple and preliminary analysis by the use of multifrequency EBI’s measurements to detect amplicons of gene-expression in final Transcriptase Reverse Polymerase Chain Reaction (RT-PCR) products, and the identification of a potential sensitivity threshold point in a specific EBI’s frequency and parameter. Final RT-PCR products for two genes amplification were analyzed by EBIs in a broad frequency range of 100 Hz to 10 MHz. Relative gene expression values were correlated with bioimpedance measurements at every frequency point. The findings suggest a relative gene over-expression threshold sensitivity point in the limit of 0.3, particularly in the phase parameter at 10 MHz.

 

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